In the present study, derivative spectrophotometric method in methanol has been developed for the determination of nadolol in bulk and pharmaceutical dosage drug. The first, second and third order derivative spectroscopy was applied for the estimation of nadolol using zero-crossing method. Nadolol exhibits absorption maxima at 270 nm. In the first, second and third derivative spectra of nadolol the amplitude of positive maxima was measured at 266 nm, 271 nm and 236 nm respectively. Linearity in the concentration range was found to be 50-250 μg/ml. The method were found to be simple economical accurate and reproducible and can be adopted in routine analysis of nadolol in bulk and dosage form.
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